Fear not. Living abroad has not turned me into an October caroler. In fact, I remain unhealthily elated at the prospects of skipping Christmas altogether this year. (My grinch-tendencies will be elucidated in a Holiday post) No, my title is referencing the emotions which surround running gel electrophoresis. Not even kidding.
I’ve detailed the “bench-high” that I experience in the lab, but the excitement-wonder-anticipation emotion amalgamation that surrounds running a gel is on another emotional plane entirely. First, there is the preparation of the PCR mixtures and the 4-hour thermo-cycler run. This step requires attention to detail, proper technique, and patience. Much like a Santa-obsessed child minding their actions and wriggling in excitement on December 1st, I usually get a little antsy during this process. I double check my PCR cocktail calculations, obnoxiously re-check that the thermo-cycler is running properly, and busy my hands on lab maintenance (or blog writing) during the wait.
Then comes the making, setting and loading the gel. This is basically like wrapping a Christmas present... In theory, the gift will appear to be more thoughtful, grander, and more sincere the nicer the wrapping technique. At least, that’s the case with gels (I contend that the wrapping quality is irrelevant at Christmas or Birthdays). Therefore, I am a stickler for proper loading technique. This is when my OCD tendencies really rear their Type-A head... I aim for perfectly straight lanes, edges free of bubbles or anomalies, and evenly filled wells.
The overwhelming anticipation/excitement of Christmas morning comes as the gel runs. For 35 minutes, which seem like hours when you are anticipating really, really good presents er.. bands, I have to sit and wait for the DNA to creep down the agarose. It reminds me of sitting at my grandparent’s breakfast table, my 5-year-old self ready to tear through the pile of presents visible in the living room, only to be told to sit up straight and finish my grapefruit. I have to pretend like I am cool-as-a-cucumber during this time when, in reality, all I can think about is whether or not there is some hidden gem of a band waiting to reveal itself to me.
Finally, I get to dig into the Gel Electrophoresis goodies. Just as some people gasp in excitement while tearing into the wrapping paper of their Christmas presents, I hold my breath as the UV light flutters onto full power and illuminates my gel. I am quite happy if I see bands that I expected to see (in the case of DNA quality checks). These bands prove that my extraction and PCR protocol is valid and that I didn’t screw up along the way. Nice to have (a real drag when they are not there), but these are not nearly as exciting as having a pathogen band show up. These bands are the equivalent of getting a present that I wanted but didn’t expect. This is usually the point when I do a happy dance, let out a yelp of glee, and smile so hard I am sore the next day.
Friday, October 29, 2010
It’s beginning to look a lot like Christmas...
Posted by Kathryn at 5:46 PM 0 comments
Labels: Fulbright, laboratory friends, Mahidol, Research Project, Thailand
I don’t like to make generalizations BUT
...after 2 months of daily field research and tedious observation I have deduced that most Thais have no spatial awareness or, for that matter, an ounce of spatial courtesy. Period.
Really, it must be genetic. Or, maybe, it is only a by-product of coping with life in the sprawling sea of people known as Bangkok. Either way, annoying doesn’t even begin to cover it.
I must be exaggerating, right? Surely, I have just run across a chance few people who were so into their moment that they failed to notice anyone else around them. Wrong. It happens constantly. There is the 20-something lady at the bottom of the escalator who stops, dead center of the landing pad, to answer a text when there are 40 steps full of people barreling down towards her. Then, there are the people who jump enthusiastically into the sidewalk bustle only to walk achingly slow and zigzag across the entire thoroughfare. These same people also like to admire the street vendors wares at random and from a distance, standing firmly in the center of the sidewalk and causing enormous people-jams. There is the very common breed who push their way onto the BTS train or bus, elbowing you 5 times on the way in and using you as a balancing prop when the ride gets rough. Better yet, there are those people who start climbing up the perilously steep bus steps as you are trying to step down, ramming their way by and nearly sending you crashing to the pavement. Love them all.
However, my absolute favorite variety of the spatially unaware emerges when it rains. They wield umbrellas and are the epitome of their kind. Being about 6” or more taller than the average Thai woman, my head exists at the height of where the pokiest part of their umbrella’s fall. If humidity is approaching the dew point umbrellas come out in full force and I am left dodging and weaving in a pitiful attempt to escape unscathed. Often, they gingerly spin their umbrellas, turning them into blades of death sure to snag into my ponytail, scratch my arms and come thisclose to leaving me with an eye patch. Matters become even more comical, or infuriating, when it actually starts to rain. People will stand under the bus stop (which has a roof, mind you) with their umbrellas deployed, tilting them back to allow a view of the approaching bus numbers and leaving those behind them (often me) drenched from the run-off and unable to view the numbers for themselves. Better yet, people actually attempt to board the bus with their umbrellas open in hopes of sparing their hair the two-seconds worth of rain they would be subjected to if they had the courtesy to close them beforehand. I couldn’t count on my fingers and toes the number of times I have been behind someone who insists on keeping their umbrella open until the very last moment... leaving me at the receiving end of the umbrella spines and the poof of water that they emit when shaking it off on the bus steps. (For these reasons I think people who choose to carry umbrellas should be subject to a permitting process complete with vision test, spatial awareness check, and a ‘driving-test’ in real world conditions. If you can’t be courteous then you shouldn’t get the benefit of staying dry.)
To be very honest, the spatial awareness disconnect is probably the issue that most aggravates me on a day-to-day basis. 98% of the time I can laugh it off. But there are the rare moments when I allow myself to get so frustrated that I just need to escape. These are the days when I forgo the bus stop, flag a taxi home from work when it is raining (sianara, umbrella-wielders!), have dinner delivered to my apartment (haha, take THAT you sidewalk hoggers!), and have a mental-health evening fit with a face-mask, hot-shower, and indulgence in the latest episode of ANTM.
Posted by Kathryn at 5:25 PM 0 comments
Labels: Bangkok, thai people, Thailand
Suu-ay Mak! Thai Standards of Beauty from a Farang Perspective.
Portion of the the "Beauty" aisle in Big C. All whitening.
Posted by Kathryn at 4:52 PM 0 comments
Labels: Bangkok, beauty, Fulbright, Mahidol, man's city, optimism, smiles, thai people, Thailand
Wednesday, October 20, 2010
Homesickness? No. Catsickness? Yes!
Posted by Kathryn at 9:28 PM 0 comments
Bliss Filled Weekend::: Amphawa, Samut Songkhram, Samut Sakhon
Posted by Kathryn at 7:38 PM 0 comments
Labels: adventures outside Bangkok, adventurous eating, Amphawa, Bangkok, Chalao, food, Fulbright, gratitude, laboratory friends, Thailand
Tuesday, October 19, 2010
Chalao's Birthday!
Posted by Kathryn at 11:30 PM 0 comments
Labels: Bangkok, Birthdays, Chalao, food, Fulbright, laboratory friends, Mahidol, Research Project, smiles, Thailand
Thursday, October 14, 2010
City Escape: Ruins, Monkeys, and Railways
Posted by Kathryn at 3:32 PM 0 comments
Labels: adventures outside Bangkok, adventurous eating, Fulbright, Lopburi, thai people
Friday, October 8, 2010
Bangkok Drag
I've been slacking in the pictures department so I just had to share these incredible shots of a drag show that I had the pleasure of attending last weekend. Frankly, I can't think of a more fabulous way to spend a Friday evening. Too much fun.
Posted by Kathryn at 11:53 PM 0 comments
Labels: Bangkok, drag show, gay bar, man's city, thai people, Thailand
I Sold Out
Posted by Kathryn at 11:42 PM 0 comments
Labels: Bangkok, gratitude, Krispy Kreme, laboratory friends, Research Project
Aftermath of a Tragedy
Posted by Kathryn at 11:18 PM 0 comments
Labels: Death, gratitude, motorcycle taxi, navigating the city, optimism, thai people, Thailand